Flow cytometry studies the optical parameters emitted by particles (cells, cell fractions). Flow cytometers can study a series of individual particle parameters simultaneously, rapidly, and over a large number of individualized particles in suspension. Flow cytometers use lasers as a source of light excitation; therefore, it must be possible to label the particles with one or more fluorescent substances. Information is also collected on the size and structural complexity of each particle. This multiparametric study of each particle allows us to analyze subpopulations in complex samples using an electronic classification. Some cytometers are also equipped with modules for classifying and collecting particles of interest.
The most important applications of flow cytometry include those related to the study of cell surface receptors, nuclear and cytoplasmic antigens, DNA content, enzymatic activity, cell integrity, and membrane permeability and flow. kicks. The cytometry platform has a wide range of flow cytometry and cell separation applications and develops or implements new ones depending on the demand of researchers. Its use has become widespread in the field of biomedicine (Hematology, Immunology, Pharmacology, Oncology, Microbiology, Genetics, etc.).
Miriam Ejarque CV
2012 – PhD in Biomedicine. Hospital Clínic-IDIBAPS at the University of Barcelona (UB), Barcelona, Spain.
2013-2014 Postdoc at Institute of Genetics and Molecular and Cellular Biology (IGBMC), Strasbourg, France.
Since 2014 Researcher at Institut d’Investigacions Sanitàries Pere Virgili (IISPV), Tarragona, Spain.
Since 2015 Head of Flow Cytometry Core Facility at IISPV, Tarragona, Spain.
The platform has a FACS Aria III (BD), a flow cytometer analyzer and a separator of particles and cells of high speed and resolution. The current configuration has 4 lasers with a total of 18 fluorochromes. In addition, it also has an autoMACS magnetic cell separator. The analysis software is FACSDiva (BD) and FlowJo. The cell classifier-separator is managed by facility personnel, who also support users in experimental design and data analysis. Analyzers can be handled by the users themselves after a training session. Installation staff assists with experimental design and oversees experiments when necessary. We recommend that you discuss the experiments with the facility staff in advance to properly design the experiments.
Cell subpopulation analysis
Apoptosis
Cell cycle, proliferation
Functional analysis
Viability
Cell separation
Advice and training for users of the platform
For more information, contact Miriam Ejarque.
A/e: citometria@iispv.cat
Institut d’Investigació Sanitària Pere Virgili
Parc Sanitari Joan XXIII
C/ Doctor Mallafrè Guasch, 4
43005 Tarragona
La Plataforma de Suport Estadístic és una plataforma transversal de suport estadístic a l’investigador que ofereix un acompanyament en totes les fases del desenvolupament d’un projecte de recerca: disseny, anàlisi, difusió i publicació.
The Clinical Pharmacy Department is a central clinical support service with its own activities and support for the other healthcare services of the ICS Camp de Tarragona.
Bioinformatics service available to all IISPV professionals.
The IISPV Biobank is a biological sample bank service
La Plataforma de Suport Estadístic és una plataforma transversal de suport estadístic a l’investigador que ofereix un acompanyament en totes les fases del desenvolupament d’un projecte de recerca: disseny, anàlisi, difusió i publicació.
The Clinical Pharmacy Department is a central clinical support service with its own activities and support for the other healthcare services of the ICS Camp de Tarragona.
Bioinformatics service available to all IISPV professionals.
The IISPV Biobank is a biological sample bank service
